Metabolomic Analysis of Biosynthesis Mechanism of ?-Polylysine Produced by Streptomyces diastatochromogenes

?-Polylysine (?-PL), a natural preservative with broad-spectrum antimicrobial activity, has been widely used as green food additive, and it is now mainly produced by Streptomyces in industry. In the previous study, strain 6#-7 of high-yield ?-PL was obtained from original TUST mutagenesis. However, biosynthesis mechanism still unclear. this metabolomic analyses both strains are investigated. Results show that difference metabolisms between significant. Based on results enzymatic activities, metabolic regulation revealed. The transport absorption capacity for glucose improved. activity benefits synthesis, such pyruvate kinase aspartokinase, strengthened. On contrary, homoserine dehydrogenase branched-chain pathways decreased. Meanwhile, increase trehalose, glutamic acid, etc. makes more resistant to ?-PL. Thus, ability mutagenized synthesize enhanced, can produce ?-PLs compared strain. For first time, analysis investigated, possible then These findings provide theoretical basis further improving production